3DLife

3DLife

Emulating life in 3D with digital and experimental tissue models.

Project overview

Project lead: Berit Løkensgard Strand
Institution: NTNU
Duration: 2017–30/6/2021

Publications

 

  • Solbu, Anita Akbarzadeh; Caballero, David; Damigos, Spyridon; Kundu, Subhas C.; Reis, Rui L. & Halaas, Øyvind [Show all 8 contributors for this article] (2023). Assessing cell migration in hydrogels: An overview of relevant materials and methods. Materials Today Bio. 18. doi: 10.1016/j.mtbio.2022.100537. Full text in Research Archive
  • Hesjedal, Maria Bårdsen (2022). Socializing Scientists into Interdisciplinarity by Placemaking in a Multi-sited Research Center. Science, Technology and Human Values. ISSN 0162-2439. doi: 10.1177/01622439221100867.
  • Akbarzadeh, Anita; Koernig, Andre; Kjesbu, Joachim Sebastian; Zaytseva-Zotova, Daria; Sletmoen, Marit & Strand, Berit Løkensgard (2021). High resolution imaging of soft alginate hydrogels by atomic force microscopy. Carbohydrate Polymers. ISSN 0144-8617. 276. doi: 10.1016/j.carbpol.2021.118804.
  • Arlov, Øystein; Rütsche, Dominic; Asadi Korayem, Maryam; Öztürk, Ece & Zenobi-Wong, Marcy (2021). Engineered Sulfated Polysaccharides for Biomedical Applications. Advanced Functional Materials. ISSN 1616-301X. 31(19), p. 1–52. doi: 10.1002/adfm.202010732.
  • Omtvedt, Line Aanerud; Kristiansen, Kåre Andre; Strand, Wenche Iren; Aachmann, Finn Lillelund; Strand, Berit Løkensgard & Zaytseva-Zotova, Daria (2021). Alginate hydrogels functionalized with β-cyclodextrin as a local paclitaxel delivery system. Journal of Biomedical Materials Research. Part A. ISSN 1549-3296. 109(12), p. 2625–2639. doi: 10.1002/jbm.a.37255. Full text in Research Archive
  • Victoria, Moreno-Manzano; Zaytseva-Zotova, Daria; López-Mocholí, Eric; Briz-Redón, Álvaro; Strand, Berit Løkensgard & Serrano-Aroca, Angel (2020). Injectable Gel Form of a Decellularized Bladder Induces Adipose-Derived Stem Cell Differentiation into Smooth Muscle Cells In Vitro. International Journal of Molecular Sciences. ISSN 1661-6596. 21, p. 1–14. doi: 10.3390/ijms21228608. Full text in Research Archive
  • Dalheim, Marianne Øksnes; Omtvedt, Line Aanerud; Bjørge, Isabel M.; Akbarzadeh, Anita; Mano, Joao F. & Aachmann, Finn Lillelund [Show all 7 contributors for this article] (2019). Mechanical Properties of Ca-Saturated Hydrogels with Functionalized Alginate. Gels. ISSN 2310-2861. 5(2), p. 1–19. doi: 10.3390/gels5020023. Full text in Research Archive

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  • Haslene-Hox, Hanne; Chahal, Aman Singh; Arlov, Øystein; Øynebråten, Ingrid Ryge; Zaytseva-Zotova, Daria & Vikenes, Anette [Show all 16 contributors for this article] (2023). Alginate hydrogel characteristics regulate fibroblast biology.
  • Zaytseva-Zotova, Daria; Haslene-Hox, Hanne; Arlov, Øystein; Hoel, Andrea Draget; Mjelle, Robin & Strand, Wenche [Show all 13 contributors for this article] (2023). Alginate hydrogel characteristics regulate NHDF biology.
  • Hoven, Sigrid Setsaas; Haslene-Hox, Hanne; Chahal, Aman S; Goksøyr, Anders; Routti, Heli Anna Irmeli & Strand, Berit Løkensgard (2022). Establishing robust 2D cultures of killer whale primary cells: an optimization study .
  • Chahal, Aman S; Strand, Berit Løkensgard; Haslene-Hox, Hanne & Sætrom, Pål (2022). An out of body experience: Cell culture in 3D.
  • Strand, Berit Løkensgard (2021). Enzymatic and chemical tailoring of alginates hydrogels for biomedical applications.
  • Zaytseva-Zotova, Daria; Haslene-Hox, Hanne; Arlov, Øystein; Vikenes, Anette; Hoel, Andrea Draget & Chahal, Aman S [Show all 15 contributors for this article] (2021). Alginate Hydrogel Characteristics Regulate Dermal Fibroblasts Biology.
  • Zaytseva-Zotova, Daria; Haslene-Hox, Hanne; Arlov, Øystein; Vikenes, Anette; Hoel, Andrea Draget & Chahal, Aman S [Show all 13 contributors for this article] (2020). Varying single parameters of the alginate hydrogels to design better microenvironments for fibroblasts cultivation in vitro.
  • Zaytseva-Zotova, Daria; Strand, Wenche Iren; Akbarzadeh, Anita; Rouhola, Rita; Haslene-Hox, Hanne & Arlov, Øystein [Show all 11 contributors for this article] (2019). Optimizing alginate gelation conditions for 3D cell culture.
  • Zaytseva-Zotova, Daria; Ruohola, Rita; Strand, Wenche Iren; Akbarzadeh, Anita; Haslene-Hox, Hanne & Arlov, Øystein [Show all 14 contributors for this article] (2019). Alginate hydrogels with tailored mechanical and biological properties for 3D fibroblast culture .
  • Zaytseva-Zotova, Daria; Ruohola, Rita; Strand, Wenche Iren; Akbarzadeh, Anita; Haslene-Hox, Hanne & Arlov, Øystein [Show all 12 contributors for this article] (2019). Soft alginate hydrogels modified with bioactive peptides as extracellular matrices for 3D fibroblast culture.
  • Hoel, Andrea Draget; Haslene-Hox, Hanne; Arlov, Øystein; Klinkenberg, Geir; Vikenes, Anette & Åslund, Andreas [Show all 11 contributors for this article] (2019). 3DLife: Validating new high-throughput methods for 3D cell culture screening.
  • Strand, Berit Løkensgard (2019). Alginate enzymatic and chemical modification and their use in biomedical applications.
  • Seres, Silvija & Strand, Berit Løkensgard (2019). #152: Vev-på-chip. [Internet]. LØRN.TECH (podcast).
  • Brautaset, Trygve; Strand, Berit Løkensgard & Tarjem, Guro (2018). Utfordringer for bioteknologien.
  • Arlov, Øystein; Haslene-Hox, Hanne; Eikenes, Åsmund H.; Carlsen, Sven Magnus & Aksnes, Astrid (2018). Tett på livet med digital forskning.
  • Halaas, Øyvind; Sætrom, Pål; Arlov, Øystein; Haslene-Hox, Hanne; Beisvag, Vidar & Aachmann, Finn Lillelund [Show all 10 contributors for this article] (2018). 3DLife – Emulating life in 3D with digital and experimental tissue models.
  • Strand, Berit Løkensgard (2018). Tissue engineering with hydrogels – building 3D tissue structures with alginate .
  • Zaytseva-Zotova, Daria; Strand, Wenche Iren; Akbarzadeh, Anita; Haslene-Hox, Hanne; Arlov, Øystein & Vikenes, Anette [Show all 10 contributors for this article] (2018). Alginate-based biomimetic matrices for 3D cell culture and high throughput screening.
  • Haslene-Hox, Hanne; Arlov, Øystein; Vikenes, Anette; Hoel, Andrea Draget; Klinkenberg, Geir & Strand, Wenche Iren [Show all 8 contributors for this article] (2018). 3D cell culture: Tailoring matrix properties for cell culture and high throughput screening.

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Research group

Cell culture-based experiments are important pillars in all medically related research, allowing examination of living cells without the use of research animals or human subjects. However, the commonly used cellular monolayer cultures are a remote reflection of in vivo conditions, due to a lack of the cellular, structural and chemical elements forming the tissue microenvironment. This disparity results in cells losing their tissue-like phenotype over time, limiting the potential of the models for studying tissue biology and disease progression, and for testing pharmaceutic and toxic compounds.

3DLife aims to develop novel strategies for microtissue engineering in 3D, to provide model systems of organ function and bridge the gap to in vivo conditions. To understand how the microenvironment affects cells we will synthesize novel and tuneable extracellular scaffold materials, and develop tools for high-throughput screening (HTS) of 3D cell cultures to assess genetic expression patterns in response to defined scaffold properties. These advances have limited translational potential without a digital approach that can process the vast data output from HTS analyses and provide a systems-level understanding of material-cell interactions. By applying a computational model, we can predict the requirements of organotypic cells to their microenvironment and tailor materials for improved in vivo-like tissue and organ models for research and clinical applications beyond the state of the art. To achieve this ambitious goal, 3DLife brings in expert competence within material engineering, high-throughput analyses, transcriptomics and bioinformatics, cell biology and cultivation, microsystem technology and mathematical and computational modelling from NTNU and SINTEF supported by international academic collaboration. The project will contribute to the Centre for Digital Life Norway (DLN) with new knowledge, materials and methodology with a broad field of application in biotechnology.

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